ABSTRACT
Objective: To use the liquid phase coprecipitation method for preparing gemcitabine magnetic nanoparticles to the pouch, and explore the preparation process in a number of conditions. Methods: The effect of different stirring speeds, Fe3+/ Fe2+ ratio, pH, temperature of Fe3O4 powder and emulsification condition, ultrasonic time, curing temperature on the preparation of targeted magnetic nanoparticles were observed. Results: The preparation of Fe3O4 nanoparticles was as follows: 800 r / min of stirring speed, 1.7: 1 Fe3+/Fe2+ ratio, pH=9, the reaction temperature of 60 °C, and the 5: 40 water / oil compared, ultrasonic time of 10 min, 100 °C curing temperature. Conclusion: Fe3O4 powder with small particle size, high purity, and no agglomeration are prepared, the stability of the gemcitabine magnetic nanoparticles capsule is good.
ABSTRACT
Objective To use the liquid phase coprecipitation method for preparing gemcitabine magnetic nanoparticles to the pouch,and explore the preparation process in a number of conditions. Methods The effect of different stirring speeds,Fe3+/Fe2+ratio,pH,temperature of Fe3O4 powder and emulsification condition,ultrasonic time,curing temperature on the preparation of target?ed magnetic nanoparticles were observed. Results The preparation of Fe3O4 nanoparticles was as follows:800 r/ min of stirring speed,1.7∶1 Fe3+/Fe2+ratio,pH=9,the reaction temperature of 60℃,and the 5∶40 water/oil compared,ultrasonic time of 10 min, 100℃curing temperature. Conclusion Fe3O4 powder with small particle size,high purity,and no agglomeration are prepared,the stability of the gemcitabine magnetic nanoparticles capsule is good.
ABSTRACT
Objective To investigate the effects of targeted treatment of the carboplatin-Fe@C nanocage-loaded chitosan nanoparticles (C-Fe@CN-CN) combining external magnetic field on rats with transplanted liver cancer.Methods Twenty-four model rats with transplanted liver cancer were established and divided into four groups randomly (n =6).Abdominal exposure was carried out through a midline incision,and a cannula was inserted into the hepatic artery and fixed.Group A:saline water was injected as control,group B:saline water with 10 mg/kg free carboplatin was given,group C:saline water with C-Fe@CN-CN (equivalent dose of free carboplatin 10 mg/kg) was injected in absence of magnetic field,group D:saline water with C-Fe@CN-CN (equivalent dose of free carboplatin 10 mg/kg) was injected in presence of magnetic field for 30 min.All the animals were sacrificed and abdominal exposure was done again after 7 days.After tumors were reselcted,tumor weight and volume was measured,the inhibiting rate of tumor weight was calculated.Tumor and liver tissues were examined for histological changes.Results The growth of tumor was significantly inhibited after therapy with different forms of carboplatin.There was significant difference in the tumor weight of A,B,C,D groups [(0.85±0.12) g,(0.61±0.10) g,(0.48±0.09) g,(0.33±0.06) g,P ＜ 0.05,respectively].The inhibiting rates of tumor weight of B,C,D groups were 28.9 ％,43.4 ％,61.7 ％ respectively.The inhibiting rate of D group was highest which was 1.1 times higher than that of B group.There was also significant difference in the tumor volume of A,B,C,D groups [(1.06±0.24) cm3,(0.72±0.10) cm3,(0.50±0.07) cm3,(0.28±0.05) cm3,P ＜ 0.05,respectively].The tumor volume of group A was largest which was 2.8 times larger than that of group D.In group D,tumor tissues from six rats presented severe necrosis,and nanoparticles were concentrated in the necrotic tissue.In group C,five rats presented middle necrosis,one rats presented severe necrosis.There was no concentration of nanoparticles in the necrotic tissue.In group B,four rats presented middle necrosis,two rats presented mild necrosis.In group A,six rats presented mild necrosis.Conclusion C-Fe@CN-C can significantly increase the therapeutic effects of carboplatin by hepatic artery injection combining with an external magnetic field on the tumor.